| 七种蟋蟀基因组DNA的RAPD多态性研究（直翅目：蟋蟀总科） |
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| 引用本文：田英芳,郑哲民.2001,七种蟋蟀基因组DNA的RAPD多态性研究（直翅目：蟋蟀总科）.Entomotaxonomia,(4): |
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| 田英芳 郑哲民 |
| [1]西安交通大学医学院法医系，陕西西安710061 [2]陕西师范大学动物研究所，陕西西安710062 |
| 基金项目:国家自然科学基金项目 (3 95 70 1 0 ) |
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| 中文摘要:应用10种随机引物，对西北地区常见的3属7种蟋蟀进行RAPD多态性检测，共筛选出2种引物S142，S8可以对7个种扩增出清晰稳定的多态性片段，多态性片段共计58条，相对分子质量在320bp-2400bp之间。应用UPGMA（非加权配对算术平均法）对多态性片段进行聚类分析，构建树状图，推测系统发生关系。每一种蟋蟀均先各自聚为一类，棺头蟋属与油葫芦属间的遗传距离最小，亲缘关系最近，斗蟋属4个种间的亲缘关系较为复杂，明显分为2个支系,与传统分类并不一致。 |
| 中文关键词:直翅目 蟋蟀总科 基因组DNA RAPD 系统发生关系 多态性 |
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| RAPD Study of Seven Species of Crickets (Orthoptera: Grylloidea) from China |
| TIAN Ying-fang 1 ZHENG Zhe-min 2 |
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| Abstract:The phylogenetic relationship of 7 species of crickets was studied by using RAPD technique.The genemic DNA of 34 samples were amplified with 10 decamer primers,only 2 primers(S142,S8) could produce clear bands.The total number of the bands are 58 among 7 species,their molecular size ranges from 320bp-2400bp.The phylogenetic relationships were inferred by using UPGMA based on RAPD makers, Individuals of the same species clustered respectively first,the genus Loxoblemmus showed close relationship with the genus Teleogryllus,the result of RAPD analysis suggest that four species of the genus Velarifietorus could be devided into two groups. |
| keywords:Orthoptera,Grylloidea,genomic DNA,RAPD,phylogenetic relationship |
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