| 中国油葫芦属昆虫的RAPD分析（直翅目：蟋蟀总科：蟋蟀科） |
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| 引用本文：李恺,郑哲民.2003,中国油葫芦属昆虫的RAPD分析（直翅目：蟋蟀总科：蟋蟀科）.Entomotaxonomia,(1): |
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| 李恺 郑哲民 |
| [1]华东师范大学生命科学学院，上海200062 [2]陕西师范大学动物研究所，陕西西安710062 |
| 基金项目:国家自然科学基金项目(3957010) |
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| 中文摘要:用RAPD技术研究了我国油葫芦属Teleogryllus 5种蟋蟀的系统发生关系。研究中使用了15个标本，在所试用的54种随机引物中，有9种引物能扩增出清晰而稳定的多态性片断，多态性片断共计167条。应用UPGMA法对多态性片断进行聚类分析，构建树状图，推测其系统发生关系。每一种蟋蟀先各自聚为一类，聚类结果所呈现的属内种间关系与传统分类研究结果基本一致，且支持黑脸油葫芦与黄脸油葫芦为独立种的观点。 |
| 中文关键词:直翅目 蟋蟀科 油葫芦属 RAPD 系统发生关系 |
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| RAPD Study of the Genus Teleogryllus (Orthoptera: Grylloidea: Gryllidae) from China |
| LI Kai 1 ZHENG Zhe min 2 |
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| Abstract:The phylogenetic relationship of 5 species of the genus Teleogryllus from China was studied by means of RAPD technique. Totals of 15 samples of Teleogryllus were used in this study. Among 54 arbitrary primers (10 bp) predetermined optimal reaction conditions, 9 primers were informative and yielded a total of 167 clear and reproducible bands. The phylogenetic relationships were inferred by using UPGMA based on RAPD makers. Individuals of the same species clustered respectively first. The result of RAPD analysis was similar to the one by using morphological characters, and agreed with the idea that T. emma (Ohmachi et Matsumura) and T. occipitalis (Serville) are two independent species. |
| keywords:Orthoptera,Gryllidae,Teleogryllus,RAPD,phylogenetic relationship |
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