| 九种蟋蟀mtDNA-16S rRNA基因序列及其系统进化(直翅目:蟋蟀科) |
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| 引用本文：蒋国芳,陈爱辉,冯金叶.2004,九种蟋蟀mtDNA-16S rRNA基因序列及其系统进化(直翅目:蟋蟀科).Entomotaxonomia,(4): |
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| 蒋国芳 陈爱辉 冯金叶 |
南京师范大学生命科学学院江苏省资源生物技术重点实验室 江苏南京210097
(蒋国芳，陈爱辉)
，南京师范大学生命科学学院江苏省资源生物技术重点实验室 江苏南京210097(冯金叶)
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| 基金项目:国家自然科学基金资助项目(30160015) |
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| 中文摘要:蟋蟀科5属9个种的线粒体16S rRNA基因部分序列被测定或从Gen Bank获得，比较其同源性，计算核苷酸使用频率，并构建NJ和MP分子系统树。在获得的449bp的序列中A、T、C和G碱基含量分别为31．8％、36．9％、9．9％和21．4％，A T平均含量为68．7％。研究结果表明：所研究的5属9种蟋蟀聚成3个聚类簇，斗蟋属先与灶蟋属汇合，再与棺头蟋属构成聚类簇Ⅰ；油葫芦属黑脸油葫芦和北京油葫芦与蟋蟀属的家蟋相聚构成聚类簇Ⅱ；蟋蟀属的田蟋单独构成聚类簇Ⅲ。 |
| 中文关键词:蟋蟀科 直翅目 系统进化 分子系统树 rRNA基因 序列 碱基 葫芦 同源性 含量 |
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| Phylogenetic Relationships of Nine Crickets Based on Partial Sequences of 16S rRNA Gene(Orthoptera:Gryllidae) |
| JIANG Guo-fang CHEN Ai-hui FENG Jin-ye |
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| Abstract:The partial sequences of mitochondrial 16S rRNA gene (449bp) were sequenced for 9 species of crickets belonging to 5 genera of Gryllidae. The homologous sequences were compared, the used frequency of nucleotide was calculated and the molecular trees reconstructed by Neighbor-Joining method and maximum parsimony method using Dianemobius taprobanensis as outgroup. In the obtained sequences of crickets, the content of A, T, C and G was 31.8%, 36.9%, 9.9% and 21.4% respectively, and A% + T% was 68.7%. The reseach results suggested that 9 individuals from the 5 genera clustered in 3 clusters, among which genus Velarifictorus and Gryllodes firstly clustered and then together with genus Loxoblemmus to form cluster I; Cluster II was made of three species, Teleogryllus emma, T. mitratus and Gryllus domesticus; G. campestris single as cluster III. |
| keywords:Orthoptera,Gryllidae,16S rRNA gene,phylogeny, |
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