红足壮异蝽Urochela quadrinotata Reuter线粒体基因组的测定与分析 |
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引用本文:戴宇婷,李 虎,姜 培,宋 凡,叶 子,袁向群,戴 勋,常 剑,彩万志.2012,红足壮异蝽Urochela quadrinotata Reuter线粒体基因组的测定与分析.Entomotaxonomia,34(4):613~623 |
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中文摘要:研究测定并分析了红足壮异蝽Urochela quadrinotata Reuter的线粒体基因组全序列。该线粒体基因组全长16585 bp (GenBank登录号为JQ743678), A+T含量为75.4%,共编码35个基因,包括13个蛋白质基因、20个tRNA基因(两个tRNA基因,即tRNAIle和tRNAGln未被检测到)、2个rRNA基因及一段较长的非编码区(控制区,亦称A+T富含区)。基因排序与大部分昆虫的线粒体基因排列方式相同,没有发生基因重排。除tRNASer(AGN)的DHU臂无法形成典型的茎环结构,其余tRNA基因均能稳定形成典型的三叶草二级结构。预测了红足壮异蝽16S和12S rRNA的二级结果,分别包括6个结构域43个茎环和3个结构域27茎环。控制区含一个长1652 bp的串联重复区域,由16个串联重复单元组成。 |
中文关键词:异蝽 线粒体基因组 RNA二级结构 |
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Sequence and organization of the mitochondrial genome of an urostylidid bug, Urochela quadrinotata Reuter (Hemiptera: Urostylididae) |
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Abstract:The complete mitochondrial genome of Urochela quadrinotata Reuter was sequenced and analyzed. This represents the first sequence completed for an insect in the family Urostylididae. The genome is 16585 bp in size and contains 35 genes (including 13 protein-coding genes, 20 transfer RNA genes, two ribosomal RNAs and a control region) with an A+T content of 75.4%. Two tRNA genes, tRNAIle and tRNAGln, are not detected in this genome. The gene order is similar to that most commonly found in insects. All tRNAs possess the typical clover-leaf structure, except tRNASer (AGN) , in which the dihydrouridine (DHU) arm forms a simple loop. The secondary structure of rrnL consists of six structural domains and 43 helices, and the rrnS includes three structural domains and 27 helices. An obvious feature of the control region is the presence of 1652 bp long tandem repeat sequences comprising 16 tandem repeat units. |
keywords:Urostylidid, mt genomics RNA second structure |
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